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| Article Name : | | | L- CARNOSINE EYE DROPS AMELIORATES LENS PROTEINS CONFORMATIONAL CHANGES AND OPACIFICATION INDUCED BY Na SELENITE: AN EXPERIMENTAL STUDY IN RATS | | Author Name : | | | Amany Abdel-Gaffar , Amany H. Elshazly, Laila H. Elshazly, Abdelnaby E. Essawy and Marwa S. Hassan | | Publisher : | | | Ashok Yakkaldevi | | Article Series No. : | | | ROR-765 | | Article : | |  | Author Profile | | Abstract : | | | he purpose of the present study is to investigate the effect of topically applied L-carnosine eye drops on the lens proteins structure and cataract development in an experimental model of selenite induced cataract in rats. Forty-five rat pups aged 13 days were used. They were randomly divided into three equal groups as follows: Group I pups received saline eye drops twice daily (control). Group II pups, (untreated cataract model), received a single subcutaneous injection of sodium selenite (19µmol/kg). Whereas group III rat pups were injected with Na selenite and received one drop of 5% L-carnosine eye drops twice daily. Animals were subjected to ophthalmological examination weekly for three weeks, then sacrificed and their lenses were prepared for biochemical analysis of catalase, superoxide dismutase, reduced glutathione, and malondialdehyde (MDA). The lens soluble proteins were analyzed and monitored by Ultraviolet (UV) absorption spectra, fluorescence spectroscopy and fluorescence of 8-anilino-1-naphthalene-sulfonic acid (ANS). Results showed significant improvement in the tested biochemical parameters including the antioxidant enzymes, proteins structural alterations. Moreover, ophthalmological examination showed marked decrease in the severity and delay in the onset of cataract in the L-carnosine treated group. In conclusion, the results of the present study showed that L-carnosine eye drops markedly ameliorated the structural lens proteins changes, lens opacification and oxidative stress in selenite-induced cataract. | | Keywords : | | - lens proteins
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