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Article Name : | | Molecular Characterization of β - subunit (accD region) of Acetyl - CoA Carboxylase gene in Microalgae | Author Name : | | SENTHIL KUMAR NATESAN , ELUMALAI SANNIYASI | Publisher : | | Ashok Yakkaldevi | Article Series No. : | | ROR-586 | Article : | | | Author Profile | Abstract : | | Acetyl – CoA Carboxylase enzyme is triggering the fatty acid biosynthesis pathway in both prokaryotic and eukaryotic cells. The enzyme consist of 4 subunits among thisBeta Subunit (accD) is the functional subunit of ACCase enzyme, which is present in plastid, transported into cytosol and assembles with other ACCase subunits such as AccA, AccBand AccC.In this study two fresh water microalgae Chlorella vulgaris, Scenedesmusobliquusand two marine microalgae Isochrysis galbana, Nannochloropsisgaditanawere taken for sequencing using designed accD primer based on already exist closest sequence of microalgae in database. Our results, suggests that the microalgae N. gaditana(738 bp)synthesize more fatty acids. It directly indicates the high level expression of accD gene due to faster transcriptional rate because of smaller gene size. The pairwise alignment showedC. vulgaris and N. gaditana had 69% similarity than other pairs. | Keywords : | | - Sequence,Sequence
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