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| Article Name : | | | RELATIVE GENE EXPRESSION STUDY USING REAL-TIME QUANTITATIVE PCR FOR GROEL GENE OF ESCHERICHIA COLI (XL1 BLUE) AND STAPHYLOCOCCUS EPIDERMIDISISOLATE | | Author Name : | | | DIXIT OJAS, RAUT AMOL, CHOUDHARY SAMEER AND KARIA JYOTI | | Publisher : | | | Ashok Yakkaldevi | | Article Series No. : | | | ROR-549 | | Article : | |  | Author Profile | | Abstract : | | | Real-Time Polymerase Chain Reaction (PCR) is highly sensitive techniques enabling amplification and quantification of a specific nucleic acid sequence with detection of the PCR product in real time. Quantitative Real-Time PCR is the conversion of the fluorescent signals from each reaction into a numerical value for each sample. A relative quantification assay is used to analyze changes in gene expression in a given sample relative to another reference sample. Real-Time PCR is the favored method for measuring gene expression. Gene expression profiling is a common type of real-time PCR assay in which the relative abundance of a transcript is assessed to determine gene regulation patterns between samples. The relative quantification of the GroEL gene varied according to the bacterial culture Species. Here, we compare GroEL gene of Staphylococcus epidermidisand Escherichia coli,used as a reference gene for gene expression measurement.The SYBR® Green-based Real-Time PCR approach used for the detection of GroEL gene expression. | | Keywords : | | - Staphylococcus epidermidis
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