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| Article Name : | | | PEROXIDASES OF SAUDI DATE CULTIVARS (PHOENIX DACTYLIFERA L.) IN TAMER STAGE | | Author Name : | | | Fahad A. Al-Jehani , Basil F. Tamim , Khalid O. Abulnaja , Ibrahim H. Kamal and Saleh A. Mohamed | | Publisher : | | | Ashok Yakkaldevi | | Article Series No. : | | | ROR-1407 | | Article : | |  | Author Profile | | Abstract : | | | Peroxidase activities were screened in sixteen cultivars of Saudi dates in tamer stage. The date cultivars with highest peroxidise activity accompanied with enhancementof the intensity of the browning which was essential character in tamer.Cationic peroxidase I from Barni was purified using ammonium sulphate, ionexchange chromatography and gel filtration. Molecular weight of peroxidase I was 64 kDa. o-Phenylenediaminewas the best substrate for the enzyme compared to other substrates. The Km values of peroxidase I for H2O2, o-phenylenediamine, guaiacol and o-dianisidine were 2.17, 5, 5.55 and 20 mM/ml, respectively. Peroxidase I was found to have temperature optimum at 40˚C and the enzyme activity was remained stable up to 40˚C. | | Keywords : | | - browning
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