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Article Details ::
Article Name :
IN VITRO ENHANCEMENT OF ANTIOXIDANT ACTIVITY, PHENOLIC CONTENT AND FLAVONOID CONTENT OF BACOPA MONNIERI L.
Author Name :
Awatef M.E. Badr-Elden.
Publisher :
Ashok Yakkaldevi
Article Series No. :
ROR-1274
Article :
Author Profile
Abstract :
Bacopa monnieri is a highly vulnerable medicinal plant from the Scorphulariaceae family. It is used as medicinal purpose and main constituent in different pharmaceutical products. The report her for the first time an enhanced Bacopa monnieri shoot for production of some metabolites. Proliferated shoots on MS medium contained some sugar (sucrose, fructose mannitol and sorbitol) and growth retardants (ABA, CCC and PBZ) are using for an enhanced factors for production of antioxidant activity, phenolic content and flavonoid content were studied. Maximum shoots obtained on MS medium supplemented with 1.5mg/l BA where, 1mg/lBA was to be superior to give maximum leaves. Height shoot length was observed on MS medium free hormone. Maximum antioxidant activity enhanced by in vitro proliferated shoots when cultured on MS medium containing 50g/l sucrose; 40g/l mannitol and 200µl/l from extracts (97.81 and 97.43% respectively). The plant extract showed significant antioxidant activitywhen shoots culturedon MS medium supplemented with0.25, 0.50, 0.75 and 0.1PBZ or ascorbic acid (positive control) with 200µl of extract. Maximum phenolic content recorded and enhanced whenshootscultured on MS mediumsupplemented with 50mg/l sucrose (14.35mg GAE/g explant) than other concentrations of fructose, mannitol and sorbitol.
Keywords :
 
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