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| Article Name : | | | BIODEGRADATION OF AZO DYE USING PEROXIDASE ENZYME EXTRACTED FROM MOSSES & CHARACTERIZATION OF DEGRADED PRODUCT | | Author Name : | | | PAWAR P. R. , LOLAGE Y .P. , MD. SAIFUL ARFEEN AND BHOSLE A.B. . | | Publisher : | | | Ashok Yakkaldevi | | Article Series No. : | | | ROR-370 | | Article : | |  | Author Profile | | Abstract : | | | Peroxidase enzyme from mosses was extracted and purified by using 70% ammonium sulphate precipitation. In burette, it was found that crude sample of Rhincostagella contains more proteins as compared to Poheli and Plagiominium. The maximum absorbance of enzyme was noted at 470 nm, which confirms the isolated enzyme is peroxidises. Guaiacol showed maximum reactivity in staining activity. Optimum temperature and pH were found to be 30oC and 7oC respectively. It was observed that all metals have inhibitory effect on enzyme activity except ZnSO4 and cystein. Beta mercaptoethanol showed maximum inhibition while ystein shows least inhibition while ZnSO4 and cystein showed Inducers effect at 1mM concentration of enzyme. As the substrate concentration increases the specific activity of enzyme also increased. The decolourization of malachite green with peroxidases was recorded up to 70 to 85 %. The degradation was monitored by UV-Visible spectrophotometer, GC-MS. | | Keywords : | | - Mosses, Peroxidases enzyme, Purification, Reactivity, Dye decolourization etc.,Mosses, Peroxidases enzyme, Purification, Reactivity, Dye decolourization etc.
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